NatureServe operates as a 501(c)3 nonprofit organization under the direction of a Board of Directors, whose members include both Network and at-large representatives.
- Nicole Firlotte - Manitoba, Canada (Chair)
- Larry Master - New York (Vice Chair)
- Sabra Tonn - Arizona (Vice Chair)
- Mary Ann Lawler - North Carolina (Treasurer)
- J. Douglas Ripley - Arizona (Secretary)
- Marcia Angle - North Carolina
- Sayles Braga - New York
- James Brumm - New York
- DJ Evans - New York
- Robert Hoguet - New York
- Urban Lehner - Washington, D.C.
- Kimberly Nelson - Washington, D.C.
- Dick Raines - Virginia
- Aníbal Ramírez Soto, Veracruz
- Alberto Székely, Mexico
- Candace Taylor-Anderson - North Carolina
- James Thorsell - Alberta
- Steven P. Quarles - Maryland
NatureServe Canada is an independent national organization based in Ottawa, Ontario. Read more about NatureServe Canada's Board of Directors.